Core Services
Service |
Detailed Service Description |
| CTC Facility Use | By reserving an available Biosafety Cabinet (BSC) on NSU’s Shared Resources Scheduler, the user is also entitled to the use of refrigerator/freezer for reagent storage, centrifuges, pipettes, light microscopes, cell counters**, and unlimited incubator space***. |
| Cryostorage - Box/Month | Cryostorage of one appropriately sized cardboard box in liquid nitrogen for a (1) month period. Note that continuous storage of samples (e.g. biobanking of cell line passages) will incur additional charges as sample volume increases over time. |
| PBMC Isolation - Tumor Matched | Isolation of PBMCs from up to 5 ml of whole blood using density-gradient centrifugation, cell count and preparation for storage in liquid nitrogen. This service does not include grant-funded technician fees or further processing to isolate specific cell populations. |
| Exosome Collection - Transfer Media | Collection, centrifugation and filtration of transfer media from incoming solid tumor sample prior to dissociation for the purposes of collecting exosomes for further purification. |
| Exosome Collection - Tumor Cells | Service includes expansion of tumor cells in a T75 flask to confluency, media change to phenol red-free culture media containing exosome-depleted FBS, culture for 24 hours prior to collection of 20 mL of culture supernatant, and media refresh for a second collection 24 hours later. Centrifugation and filtration of culture supernatant from both collections, freezing and storage of culture supernatant until further purification of exosomes, and end point cell count. |
| PBMC Isolation - Healthy Donor | Preparation of 50 million fresh or previously frozen PBMCs isolated from healthy donors using density-gradient centrifugation. This service does not include further processing to isolate specific cell populations. |
| Lentivirus Production - Small | Calcium Phosphate transfection of HEK-293FT cells with gene of interest plasmid (must be provided by user) in LeGO backbone and packaging plasmids. Collection and filtration of 10 mL of supernatant from 24 and 36 hour time points (20 mL total volume). This service does not include further purification, concentration, titration or target cell transduction. Lentivirus production systems other than LeGO can be accommodated upon request but may incur additional charges. Please contact the CTC Lab Manager for further details. |
| Lentivirus Production - Large | Calcium Phosphate transfection of HEK-293FT with gene of interest plasmid (must be provided by user) in LeGO backbone and packaging plasmids. Collection and filtration of 25 mL of supernatant from 24 and 36 hour time points (50 mL total volume). This service does not include further purification, concentration, titration or target cell transduction. Lentivirus production systems other than LeGO can be accommodated upon request but may incur additional charges. Please contact the CTC Lab Manager for further details. |
| Lentivirus Production - Titer | Determination of virus titer (infectious units per mL) by 4-point dilution series in HEK-293FT cells using flow cytometry (additional charges based on use of the flow core will apply). The virus must transduce a detectable fluorescent molecule. |
| Researcher/PI/Month - Custom Culture | Custom culture charges for (1) billing cycle. Charges will include reagents and consumables used during the billing cycle and applicable technician fees. Fees for use of the CTC facility will be charged separately (for additional details and cost see Service 1. CTC Facility Use – Hour). |
|
Akta Use Name/Month -Custom |
Custom charges for (1) billing cycle. Charges will include use of the instrument (actual usage billed hourly), a “per-run” fee for use of supplied purification column (no charge if user supplies the purification column), and applicable technician fees. All other reagents and consumables must be supplied by the user. Please note that only trained users are permitted access to the Akta M1 instrument. Please contact the CTC Lab Manager for further details. |
| Nanosight Use - Name/Month -Custom | Custom charges for (1) billing cycle. Charges will include use of the instrument (actual usage billed hourly) and applicable technician fees. All other reagents and consumables must be supplied by the user. Please note that only trained users are permitted access to the Nanosight instrument. Please contact the CTC Lab Manager for further details. |
| Solid Tumor Dissociation | Dissociation of solid tumor material includes (1) reaction of dissociation enzymes, filtering, counting, and initial plating of cells in tumor complete growth medium. This service includes applicable technician fees; but does not include further processing of dissociated tumor to obtain specific cell populations. |
| NK Cell Isolation - Full | Isolation of NK Cells from 100 million freshly isolated or frozen PBMCs using the Miltenyi Human NK Isolation Kit reagents on the AutoMACS Pro instrument. Service includes applicable technician fees. Please note that NK cell yields are donor dependent and cannot be guaranteed. Service does not include further expansion of cells or additional experiments. |
| NK Cell Isolation - User Kit | Isolation of NK Cells from 100 million freshly isolated or frozen PBMCs using Miltenyi Human NK Isolation Kit reagents (supplied by the user) on the AutoMACS Pro instrument. Service includes applicable technician fees. Please note that NK cell yields are donor dependent and cannot be guaranteed. Service does not include further expansion of cells or additional experiments. |
| CD45 Cell Isolation - Full | Isolation of CD45 Cells from 10 million freshly dissociated tumor cells using Miltenyi Human CD45 microbeads on the AutoMACS Pro instrument. Service includes applicable technician fees. Please note that CD45 cell yields are donor dependent and cannot be guaranteed. Service does not include further expansion of cells or additional experiments. |
| CD34 Cell Isolation - Full (No CBMCs) | Isolation of CD34+ Hematopoietic Stem Cells (HSCs) from 100 million freshly isolated or frozen Cord Blood Mononuclear Cells (CBMCs) using Miltenyi Human CD34 microbeads on the AutoMACS Pro instrument. Service includes applicable technician fees. Please note that CD34 cell yields are donor dependent and cannot be guaranteed. Service does not include initial CBMC isolation (for additional details and cost see Service 26. CBMC Isolation), further cell expansion or additional experiments. |
| CD14 Cell Isolation - Full | Isolation of CD14+ Cells from 100 million freshly isolated or frozen PBMCs using the Miltenyi Human NK Isolation Kit reagents on the AutoMACS Pro instrument. Service includes applicable technician fees. Please note that CD14 cell yields are donor dependent and cannot be guaranteed. Service does not include further expansion of cells or additional experiments. |
| Cell Culture Passage - DMEM/T75 | Service includes (1) passage of adherent cells cultured in DMEM Complete Medium in a T75 flask (e.g. HEK-293FT cells) and applicable technician fees. Depending on cell growth, cell lines typically require 3 passages per week of culture. |
| Cell Culture Passage - RPMI/T75 | Service includes (1) passage of adherent cells cultured in RPMI Complete Medium in a T75 flask (e.g. A375 cells) and applicable technician fees. Depending on growth rate, cell lines typically require 3 passages per week of culture. |
| Cell Culture Passage - Tumor/T75 | Service includes (1) passage of adherent tumor-derived cells cultured in Tumor Complete Medium in a T75 flask and applicable technician fees. Depending on growth rate, cell lines typically require 1-2 passages per week of culture. |
| Training Tier I | Orientation for access and independent culture work within the Cell Therapy Core Facility. |
| Training Tier II | Service includes (1) hour of customized training for projects performed within the CTC. |
| CBMC Isolation | Preparation of 50 million fresh or previously frozen Cord Blood Mononuclear Cells isolated from healthy donors using density-gradient centrifugation. This service does not include further processing to isolate specific cell populations. Please contact the CTC Lab Manager for cost and further details. |
* For pricing please contact NSUCTC@nova.edu
**Use of automated cell counters requires a cell counting chamber, charged separately
***Unlimited incubator use is subject to change depending on volume of work and number of researchers using CTC